Chiral Chromatography

Chiral Chromatography Thomas E. Beesley Advanced Separation Technologies Inc.

Chiral Chromatography

Author: Thomas E. Beesley

Publisher: John Wiley & Sons

ISBN: 9780471974277

Page: 522

View: 222

Chiral Chromatography Thomas E. Beesley Advanced Separation Technologies Inc., Whippany, New Jersey, USA Raymond P. W. Scott Chemistry Department, Georgetown University, Washington DC, USA and Chemistry Department, Birkbeck College, University of London, UK Analytical techniques based on separation processes, such as chromatography and electrophoresis, are finding a growing range of applications in chemical, pharmaceutical and clinical laboratories. The Wiley Separation Science Series provides the analyst in these laboratories with well-focused books covering individual techniques, so that they can be applied more efficiently and effectively to contemporary analytical problems. The different enantiomers of a drug can exhibit widely different physiological activity in degree and nature. As a result, the separation and identification of enantiomers is now a very important analytical problem and chiral chromatography is the natural technique to apply to the resolution of such mixtures. Chiral Chromatography provides the reader with a basic understanding of the nature of chromatographic separations and relates the principles specifically to the separation of enantiomers. The following information is included: * chiral separations involving both gas and liquid chromatography * descriptions of the apparatus used for both techniques * detailed discussion on the retention mechanism that results in chiral selectivity * the structure and synthesis of a wide range of chirally active stationary phases used in both gas and liquid chromatography * preparative applications for large scale purification of enantiomers * applications of capillary electrophoresis and capillary electrochromatography. In addition to the above, a large number of examples of the separation of both commercially and physiologically interesting chiral mixtures are given, as is a detailed discussion on the mechanism of selectivity of each example. Thomas Beesley was founder and is the CEO for a leading manufacturer of chiral stationary phases and has published papers on TLC, HPLC and chiral separations involving cyclodextrins. He has also coauthored papers with Daniel W. Armstrong, an expert on modern cyclodextrin columns. Raymond Scott has worked in the field of separation science for over 40 years and has contributed extensively to the development of both gas and liquid chromatography publishing over 160 papers on the subjects.

Thin Layer Chromatography in Chiral Separations and Analysis

A fast, inexpensive, and versatile separation technique, there are many practical considerations that contribute to its effectiveness. Thin Layer Chromatography in Chiral Separations and Analysis is the first bo

Thin Layer Chromatography in Chiral Separations and Analysis

Author: Teresa Kowalska

Publisher: CRC Press

ISBN: 0849343968

Page: 436

View: 997

Thin layer chromatography (TLC) is well suited for performing enantioseparations for research as well as larger-scale applications. A fast, inexpensive, and versatile separation technique, there are many practical considerations that contribute to its effectiveness. Thin Layer Chromatography in Chiral Separations and Analysis is the first bo

Chiral Separations By Liquid Chromatography And Related Technologies

Besides , the fate of the chiral xenobiotics is an important issue in environmental
studies . Therefore , chiral separation ( resolution ) at the analytical and
preparative scales of racemic compounds is essential and urgently needed in the
modern ...

Chiral Separations By Liquid Chromatography And Related Technologies

Author: Hassan Y. Aboul-Enein

Publisher: CRC Press

ISBN: 9780203911112

Page: 400

View: 195

Unique in its systematic and detailed description of the various types, structures, and properties of chiral stationary phases (CSPs) and their preparation, application, and future scope, this volume highlights an assortment of liquid chromatographic approaches, including sub- and super-critical fluid chromatography, capillary electrochromatography

Chiral Separations by Chromatography

The last two methods are not strictly chromatographic , but are included in this
book to provide interesting avenues for separations . Chromatographic methods
are considered most useful for enantiomeric resolution for a number of reasons ...

Chiral Separations by Chromatography

Author: Satinder Ahuja

Publisher: Amer Chemical Society

ISBN:

Page: 244

View: 632

A large number of examples are given that will assist in the selection of a method, including thin-layer chromatography, capillary electrophoresis and membrane separations." "This book will be a reliable guide for those just starting out in pharmaceutical and related industries, as well as those with experience in the field."--Jacket.

Chiral Separations

In recent years, chromatographic methods such as gas chromatography and,
especially, liquid chromatography have attracted increasing interest for chiral
separation, both on analytical and preparative scales. More recently, capillary ...

Chiral Separations

Author: Gerald Gübitz

Publisher: Springer Science & Business Media

ISBN: 1592596487

Page: 448

View: 819

Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques.

Design of Chiral Separations by Liquid Chromatography and Capillary Electrophoresis for Amlodipine and Derivatives

The design of chiral separations in liquid chromatography (LC) and capillary electrophoresis (CE) involves the selection of chiral selectors and eluent parameters, often on a purely empirical basis.

Design of Chiral Separations by Liquid Chromatography and Capillary Electrophoresis for Amlodipine and Derivatives

Author: Tracy Sharon Small

Publisher:

ISBN:

Page: 224

View: 517

The design of chiral separations in liquid chromatography (LC) and capillary electrophoresis (CE) involves the selection of chiral selectors and eluent parameters, often on a purely empirical basis. It would be desirable if rapid screening methods could be designed to rationalise the choice of these chiral selectors. With reference to the use of cyclodextrin (CD) derivatives as chiral selectors, nuclear magnetic resonance spectroscopy (NMR) can play an important role in screening the extent of interactions with chiral solutes, and in probing the nature of the stereoselective interactions involved. Data from high-field NMR on drugs and their derivatives have been explored for screening a number of potential chiral recognition agents, as an aid to the rational design of chiral separations by LC and CE, based on cyclodextrins (alpha, beta, gamma, hydroxypropyl-beta and hydroxyethyl-beta) bonded to silica (LC) and in free solution (CE). In this investigation high-field FT-NMR is used to examine the interaction mechanism between these cyclodextrins and the calcium channel blocker Amlodipine together with a series of its structural analogues. Enantiomeric interactions between the cyclodextrins and Amlodipine are explored using ROESY (rotating frame nuclear Overhauser effect spectroscopy) in order to determine the inclusion mechanism involved......

Chiral Separation Methods for Pharmaceutical and Biotechnological Products

Separation. of. Enantiomers. by. Gas. Chromatography. on. Chiral. Stationary.
Phases. VOLKER SCHURIG Institute of Organic Chemistry, University of T
̈ubingen, T ̈ubingen, Germany 1 INTRODUCTION There exist two approaches
for ...

Chiral Separation Methods for Pharmaceutical and Biotechnological Products

Author: Satinder Ahuja

Publisher: John Wiley & Sons

ISBN: 9781118097755

Page: 400

View: 207

Discusses chiral separations and offers guidance for selectingthe optimum method for desired results Chiral separations represent the most intriguing and, by somemeasures, most difficultseparations of chemical compounds. Thisbook provides researchers and students an under-standing of chiralseparations and offers a convenient route to selecting the bestseparation method, saving considerable time and cost in productdevelopment. Considering chiral separations in the biotechnological andpharmaceutical industries, as well as for food applications, Dr.Ahuja provides insights into a broad range of topics. Opening witha broad overview of chiral separations, regulatory considerationsin drug product development, and basic issues in methoddevelopment, the book: Covers a variety of modern methods such as gas chromatography,high performance liquid chromatography, supercritical fluidchromatography, and capillary electrophoresis Deals with the impact of chirality on the biological activity ofsmall and large molecules Provides detailed information on useful chiral stationary phases(CSPs) for HPLC Includes handy information on selection of an appropriate CSP,including mechanistic studies Offers strategies for fast method development with HPLC, SFC,and CE Discusses preparatory methods utilized in the pharmaceuticalindustry With in-depth discussions of the current state of the field aswell as suggestions to assist future developments, ChiralSeparation Methods for Pharmaceutical and BiotechnologicalProducts is an essential text for laboratory investigators,managers, and regulators who are involved in chiral separations inthe pharmaceutical industry, as well as students preparing forcareers in these fields.

Chiral Separation Techniques

4. Chiral. Separations. Using. Supercritical. Fluid. Chromatography. Karen W.
Phinney and Rodger W. Stringham 4.1 ... The importance of chirality in biological
activity is now well established and, as a result, chromatographic separations of ...

Chiral Separation Techniques

Author: Ganapathy Subramanian

Publisher: John Wiley & Sons

ISBN: 352761172X

Page: 641

View: 169

Thoroughly revised, with either entirely new or completely updated contents, this is a practical manual for the small and large-scale preparation of enantiomerically pure products. The result is a vital resource for meeting the highest purity standards in the manufacture of chiral pharmaceuticals, food additives and related compounds. All the approaches covered here are highly relevant to modern manufacturing and quality control schemes in the pharmaceutical and biotech industries, addressing the increasingly important issue of drug safety in view of tougher regulatory standards worldwide.

Chromatography and Separation Science

I found that I had to use at least a dozen books to convey the subject of
chromatography and separation science to the ... in HPLC (Wiley), Trace and
Ultratrace Analysis by HPLC (Wiley), and Chiral Separations by Chromatography
(Oxford).

Chromatography and Separation Science

Author: Satinder Ahuja

Publisher: Academic Press

ISBN: 9780120449811

Page: 250

View: 529

The basic objectives of this book are to: provide basic information on chromatography and separation science; show how simple extraction and partition processes provide the basis for development of chromatography and separation science; describe the role of chromatography and separation science in various fields; discuss the role of chromatography and separation science in development of new methodology; and present new evolving methods and how to select an optimum method. · The book covers the fundamental physical and chemical phenomena involved in separations · Provides a concise overview of the basics of transport phenomena and thermodynamics · Shows the importance of chromatography within separation science

Chiral Environmental Pollutants

Investigations with unmodified cyclodextrins showed that they may function as
chiral shift reagents in solution together with a racemic substrate ... König WA (
1987 ) The practice of enantiomer separation by capillary gas chromatography .

Chiral Environmental Pollutants

Author: R. Kallenborn

Publisher: Springer Science & Business Media

ISBN: 9783540664239

Page: 210

View: 236

This monograph contains a survey on the role of chirality in ecotoxicological processes. The focus is on environmental trace analysis. Areas such as toxicology, ecotoxicology, synthetic chemistry, biology, and physics are also covered in detail in order to explain the different properties of enantiomers in environmental samples. This monograph delivers a comprehensive survey for environmental trace analysts, analytical chemists, ecotoxicologists, food scientists and experienced lab workers.

Chiral Separations by Capillary Electrophoresis

47 Gerald Gübitz and Martin G. Schmid Factors Influencing Cyclodextrin-
Mediated Chiral Separations. ... 163 Vincenzo Cucinotta and Alessandro
Giuffrida Chiral Separations by Micellar Electrokinetic Chromatography.

Chiral Separations by Capillary Electrophoresis

Author: Ann Van Eeckhaut

Publisher: CRC Press

ISBN: 9781420069341

Page: 543

View: 506

Covers the Fundamentals of Chiral Separation, Available Chiral Selectors, and Numerous Applications of Chiral Separation by Capillary Electrophoresis Since the 1980s, modern analytical tools have enabled capillary electrophoresis to become a standard part of the chemist’s toolkit. With contributions from international experts, Chiral Separations by Capillary Electrophoresis provides a general overview of the principles of chiral separation by capillary electrophoresis and the different chiral selectors available. The book discusses the most important as well as several new chiral selectors used in capillary electrophoresis. It reviews recent pharmaceutical and biomedical applications and explores novel techniques, such as capillary electrophoresis coupled to mass spectrometry and microchip technology. The book also examines the quantitative aspects of capillary electrophoresis, the possibilities of capillary electrochromatography, and the various chiral columns available. Capillary electrophoresis has proven to be an effective tool for chiral separation. This book explains how this technique can be used in the separation of molecules, offering insight into both existing and emerging applications.

Chiral Separations by Liquid Chromatography

M Human serum albumin Continued competitor in mobile phase , effect on
chromatographic retention of ligand , 149 150 stereoselectivity of binding area ,
149 synthesis of CSP , 150 Hydrogen bond association , LC separation of
enantiomers ...

Chiral Separations by Liquid Chromatography

Author: Satinder Ahuja

Publisher: Amer Chemical Society

ISBN:

Page: 239

View: 125

A definitive reference for researchers working on problems involving chirality. Presenting state-of-the-art information, this volume covers all the major modes of separation and offers contributions from the leading researchers who developed these techniques. Four chapters provide a detailed review of the commonly used columns: brush type, cyclodextrin, polysaccaride carbonate, and protein. Many of the papers focus on HPLC, a technique which is also ideally suited for large scale preparation of optical isomers. In addition, the volume provides significant discussion on the use of chiral discriminators or selectors.

A Practical Approach to Chiral Separations by Liquid Chromatography

The major advantages of the sequential approach stem from the ability to use the
best possible achiral and chiral separations , even when the two modes of
chromatography have completely incompatible mobile phases ( eg , normal - and
 ...

A Practical Approach to Chiral Separations by Liquid Chromatography

Author: G. Subramanian

Publisher: Wiley-VCH

ISBN:

Page: 405

View: 791

Both analytical and preparative-scale enantioseparation techniques are covered in a down-to-earth practical way. The most important aspects of design, economics and safety are considered with emphasis on current European and North American legislation. In addition, the theory of chiral separation is covered in sufficient detail to guide the practising chromatographer interested in developing new techniques. A team of experts from academic and industrial laboratories throughout the world have compiled their findings and experience to make this book an exceptionally timely and unique contribution to the field.

Chiral Recognition in Separation Methods

The coverage in this book includes a range of separation methods, such as gas, liquid, or countercurrent chromatography, and capillary electrophoresis. The special case of chiral ionic liquids is examined in detail.

Chiral Recognition in Separation Methods

Author: Alain Berthod

Publisher: Springer Science & Business Media

ISBN: 9783642124457

Page: 337

View: 610

What drives a scientist to edit a book on a speci c scienti c subject such as chiral mechanisms in separation methods? Until December 2005, the journal Analytical Chemistry of the American Chemical Society (Washington, DC) had an A-page section that was dedicated to simple and clear presentations of the most recent te- niques or the state of the art in a particular eld or topic. The “A-page” section was prepared for a broad audience of chemists including industrial professionals, s- dents as well as academics looking for information outside their eld of expertise. 1 Daniel W. Armstrong, one of the editors of this journal and a twenty-year+ long friend, invited me to present my view on chiral recognition mechanisms in a simple and clear way in an “A-page” article. In 2006, the “A-page” section was maintained as the rst articles at the beginning of each rst bi-monthly issue but the pagination was no longer page distinguished from the regular research articles published by the journal. During the time between the invitation and the submission, the A-page section was integrated into the rest of the journal and the article appeared as (2006) Anal Chem (78):2093–2099.

Chiral Separations

This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral ...

Chiral Separations

Author: Gerhard K.E. Scriba

Publisher: Humana

ISBN: 9781493994373

Page: 487

View: 872

This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry.

Cyclodextrins in Chromatography

88) Chiral CE and HPLC methods were developed for the study of the in vitro
metabolism of the racemic antiasthmatic/antiallergic drug flezelastine.92 Human,
rat, bovine and ... Chiral separation was followed by the reversal of migration
order.

Cyclodextrins in Chromatography

Author: Tibor Cserháti

Publisher: Royal Society of Chemistry

ISBN: 9780854045402

Page: 157

View: 860

Any professional working with chromatography will welcome this unique book as both a practical compilation of methods and a source of reference to the literature.

Enantiomer Separation

Accordingly, “Enantiomer Separation” is used in the title of this book. The editor and all chapter contributors hope that this book is helpful for scientists and engineers working in this field.

Enantiomer Separation

Author: Fumio Toda

Publisher: Springer Science & Business Media

ISBN: 1402023375

Page: 333

View: 733

In spite of important advances in asymmetric synthesis, chiral compounds cannot all be obtained in a pure state by asymmetric synthesis. As a result, enantiomer separation remains an important technique for obtaining optically active materials. Although asymmetric synthesis is a once-only procedure, an enantiomer separation process can be repeated until the optically pure sample is obtained. This book discusses several new enantiomer separation methods using modern techniques developed by experts in the field. These methods consist mainly of the following three types: 1) Enantiomer separation by inclusion complexation with a chiral host compound 2) Enantiomer separation using biological methods 3) Enantiomer separation by HPLC chromatography using a column containing a chiral stationary phase. Separation of a racemic compound has been called “optical resolution” or simply “resolution”. Nowadays, the descriptions “enantiomer resolution” or “enantiomer separation” are also commonly used. Accordingly, “Enantiomer Separation” is used in the title of this book. The editor and all chapter contributors hope that this book is helpful for scientists and engineers working in this field.

Handbook of Methods and Instrumentation in Separation Science

Liquid Chromatography: Mechanisms: Chiral k 2 1⁄4 tr2 À t0 ð Þ t0 1⁄22 ... The
separation of racemic compounds into their constituent enantiomers is now
routinely performed by high performance liquid chromatography (HPLC) on chiral
 ...

Handbook of Methods and Instrumentation in Separation Science

Author:

Publisher: Academic Press

ISBN: 9780123757272

Page: 880

View: 396

Handbook of Methods and Instrumentation in Separation Science, Volume 1 provides concise overviews and summaries of the main methods used for separation. It is based on the Encyclopedia of Separation Science. The handbook focuses on the principles of methods and instrumentation. It provides general concepts concerning the subject matter; it does not present specific procedures. This volume discusses the separation processes including affinity methods, analytical ultracentrifugation, centrifugation, chromatography, and use of decanter centrifuge and dye. Each methodology is defined and compared with other separation processes. It also provides specific techniques, principles, and theories concerning each process. Furthermore, the handbook presents the applications, benefits, and validation of the processes described in this book. This handbook is an excellent reference for biomedical researchers, environmental and production chemists, flavor and fragrance technologists, food and beverage technologists, academic and industrial librarians, and nuclear researchers. Students and novices will also find this handbook useful for practice and learning. One-stop source for information on separation methods General overviews for quick orientation Ease of use for finding results fast Expert coverage of major separation methods Coverage of techniques for all sizes of samples, pico-level to kilo-level

Chromatographic Separations of Stereoisomers

Gil-Av, E., Feibush, B., and Charles-Sigler, R., Separation of enantiomers by gas-
liquid chromatography with an optically active stationary phase, Tetrahedron Lett.
, No. 10, 1009, 1966. . Liu, R. H. and Ku, W. W., Chiral stationary phases for the ...

Chromatographic Separations of Stereoisomers

Author: Rex W. Souter

Publisher: CRC Press

ISBN: 9780849361272

Page: 256

View: 173

Provides current knowledge about separation and interactions of asymmetric molecules, as well as experimental and commercial ma-terials such as columns, instruments, and derivatization reagents. Extensive applications are tabulated by both chromato-graphic technique and compound class, and discussions of recent special topics are useful in planning new work. This unique volume organizes most of the significant, currently available knowledge re-garding the chromatographic separations of stereoisomers. Both diastereomers and the more difficult, controversial area of enan-tiomers are covered in depth with respect to GC, HPLC, and classi-cal chromatographic techniques. Analytical, organic, pharmaceuti-cal, and other chemists as well as pharmacologists and bio-chemists are among those whose work appears in the more than 800 references cite.