Circulating Nucleic Acids in Plasma Or Serum

An Overview on the Isolation and Analysis of Circulating Tumor DNA in Plasma
and Serum JIN JEN , LI WU , AND DAVID SIDRANSKY Division of Head and
Neck Cancer Research , Departments of Otolaryngology and Oncology , The
Johns ...

Circulating Nucleic Acids in Plasma Or Serum

Author: Philippe Anker

Publisher:

ISBN: 9781573312691

Page: 188

View: 700

Free DNA present in both healthy and diseased human plasma has been found to express specific point mutations that may serve as diagnostic signposts - perhaps to reliable, non-invasive tests for breast and lung cancer, haematopoetic malignancies, colorectal or pancreatic carcinoma, and other tumours. In another research area, foetal DNA has been detected in maternal serum which presents an early-horizon alternative in utero test for gender. A parallel discovery of rearranged immunoglobin heavy-chain DNA in the plasma of patients wit Hodgkin's disease or acute B cell leukaemia has also emerged. These fast-emerging indicators suggest that it is time to standardize research techniques and develop protocols that can take a measure of circulating DNA's usefulness as a diagnostic tool. This volume, taken from the proceedings of a conference in August 1999, discusses such issues.

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Author: Dave S. B. Hoon

Publisher:

ISBN:

Page: 325

View: 289

Numerous questions remain regarding the origin, release, clearance, clinical utility, and possible functionality of circulating nucleic acids. Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.

Circulating Nucleic Acids in Plasma and Serum V

48 According to Bianchi et al . , 47 the ma - significantly higher circulating plasma
DNA jority of fetal DNA during pregnancy originates concentrations than in
normal controls , and from the placenta . Free DNA in the fetus may even higher ...

Circulating Nucleic Acids in Plasma and Serum V

Author: Peter B. Gahan

Publisher: Blackwell Pub

ISBN:

Page: 345

View: 900

DNA and RNA fractions have been isolated from whole blood, serum, plasma, the surface of blood cells, and urine from both healthy persons and patients. Specific fragments have been identified as being related to particular disorders, for example, diabetes, cancer, myocardial infarction, and stroke. The ability to isolate these fractions and assess their relationship to specific diseases makes early noninvasive diagnosis possible. This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter cells and exhibit biological activity, isolating and testing fetal cells from maternal blood, and discussing the clinical implications of these discoveries and developing the techniques that will bring them into clinical application. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Quantification of Circulating DNA in the Plasma and Serum of Cancer Patients
BRET TABACK , “ STEVEN J. O'DAY , AND DAVE S.B. HOON " Department of
Molecular Oncology and bCancer Institute Medical Group , John Wayne Cancer ...

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Author: Dave S. B. Hoon

Publisher:

ISBN:

Page: 325

View: 635

Numerous questions remain regarding the origin, release, clearance, clinical utility, and possible functionality of circulating nucleic acids. Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.

Journal of the National Cancer Institute

use of different procedures to quantify circulating DNA ) on the variability of
quantitative assays . ... REFERENCES NOTES ( 1 ) Anker P , Mulcahy H , Stroun
M. Circulating nucleic acids in plasma and serum as a noninvasive investigation
for ...

Journal of the National Cancer Institute

Author:

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Departments of Labor Health and Human Services Education and Related Agencies Appropriations for 2006 Department of Labor

The NCI is actively pursuing programs that support the development of new
molecular diagnostics using circulating DNA , RNA , and protein in plasma and
serum . Although the exact mechanism of how tumor nucleic acids are released
is ...

Departments of Labor  Health and Human Services  Education  and Related Agencies Appropriations for 2006  Department of Labor

Author: United States. Congress. House. Committee on Appropriations. Subcommittee on the Departments of Labor, Health and Human Services, Education, and Related Agencies

Publisher:

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Index Medicus

NUCLEOCAPSID PROTEINS PATHOLOGY BLOOD ANALYSIS Multiparameter
analysis of AgNOR in thyroid lesions : comparison Patterns of circulating hepatitis
B virus serum nucleic acids during Detection of severe acute respiratory ...

Index Medicus

Author:

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Circulating Nucleic Acids in Plasma and Serum

Proceedings of the 6th international conference on circulating nucleic acids in
plasma and serum held on 9-11 November 2009 in Hong Kong. Peter B. Gahan.
Chapter 33 Microvesicles Circulating in Plasma of Rats Contain DNA: Are These
 ...

Circulating Nucleic Acids in Plasma and Serum

Author: Peter B. Gahan

Publisher: Springer Science & Business Media

ISBN: 9789048193820

Page: 285

View: 957

DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.

Global Health and Pathology an Issue of the Clinics in Laboratory Medicine E Book

Different anticoagulants may be used, such as ethylenediamine tetra-acetic acid (
EDTA), heparin, and acid citrate dextrose (ACD). The end ... biology analyses in
the nucleic acid samples obtained either from the cellular blood fraction or
plasma itself (circulating nucleic acids). ... Preparing and storing serum instead of
plasma offers 2 advantages for a tumor bank: (1) serum does not contain
platelets and ...

Global Health and Pathology  an Issue of the Clinics in Laboratory Medicine  E Book

Author: Dan Milner

Publisher: Elsevier

ISBN: 0323581595

Page:

View: 948

This issue of Clinics in Laboratory Medicine, edited by Dr. Dan Milner, will focus on Global Health and Pathology. Topics include, but are not limited to: Clinical Laboratory Volunteerism, Pathologists Overseas; Anatomic Pathology Volunteerism; Funding Strategies in Research and Global Pathology; Diagnostics for Cancer and Health Systems building through pathology laboratories; Training the next pathologists in global health; Maximizing internet resources for improving pathology/lab medicine in LMICs; Pathology-based research in Africa; HIV and Cancer: Role of Pathology in success; Lymphoma and Pathology in Africa: Current approaches and future diagnostics; Laboratory Capacity as a tool for building health systems; Building Cross Country Networks for Laboratory Capacity and improvement; Lab accreditation; Practical success in Telepathology experiences in Africa; Pathology and WHO vision of the future of LMICs; Breast Cancer in LMICs: Why we need pathology and clinical trial capability to solve this challenge; Cytopathology in LMICs: why and how to integrate to capacitate healthcare; and Biorepositories and Data Cores for Research in Global Health.

Anticancer Research

22 Ziegler A , Zangemeister - Wittke U and Stahel RA : Circulating DNA : a new
diagnostic gold mine ? Cancer Treat Rev 28 : 255271 , 2002 . 23 Tsang JC and
Lo YM : Circulating nucleic acids in plasma / serum . Pathology 39 : 197-207 ...

Anticancer Research

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Journal of the National Cancer Institute

use of different procedures to quantify circulating DNA ) on the variability of
quantitative assays . ... REFERENCES NOTES ( 1 ) Anker P , Mulcahy H , Stroun
M. Circulating nucleic acids in plasma and serum as a noninvasive investigation
for ...

Journal of the National Cancer Institute

Author:

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View: 515

"Summaries of papers" contained in the journal accompany each issue, 19--

Annals of Clinical Biochemistry

Apoptosis is believed to play an important role in the generation of circulating
DNA in healthy subjects as well as in cancer patients . Jahr et al.41 and Giacona
et al.42 have shown that most DNA molecules present in plasma / serum have ...

Annals of Clinical Biochemistry

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Experimental Biology and Medicine

Emlen W , Mannik M. Kinetics and mechanisms for removal of circulating single -
stranded DNA in mice . ... Anker P , Mukcahy H , Chen XQ , Stroun M. Detection
of circulating tumor DNA in the blood ( plasma / serum ) of cancer patients .

Experimental Biology and Medicine

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View: 174

Salivary Diagnostics

Salivary Diagnostics

Author: David Wong

Publisher: Wiley-Blackwell

ISBN:

Page: 301

View: 825

'Salivary Diagnostics' describes the nature of saliva and the basis for its usefulness as a diagnostic fluid. It then surveys and reviews the field of research, moving on to detail the development of technologies and the deciphering of the human salivary proteome.

Genome Research

Cellular and viral DNA hypomethylation associated with induction of Epstein -
Barr virus lytic cycle . Proc . Natl . Acad . Sci . 82 : 8090-8094 . Tsang , J.C. and
Lo , Y.M. 2007. Circulating nucleic acids in plasma / serum . Pathology 39 : 197-
207 .

Genome Research

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View: 710

Proceedings of the National Academy of Sciences of the United States of America

The median number of APC DNA fragments in such patients was 47,800 per ml
of plasma , of which 8 % were mutant . ... Moreover , several studies have shown
that loss of heterozygosity is routinely detectable in circulating DNA , even in
patients with relatively nonaggressive tumors . ... Accordingly , numerous studies
have attempted to identify abnormal forms or quantities of DNA in plasma or
serum ( 6 ...

Proceedings of the National Academy of Sciences of the United States of America

Author: National Academy of Sciences (U.S.)

Publisher:

ISBN:

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View: 885

Circulating Nucleic Acids in Early Diagnosis Prognosis and Treatment Monitoring

Stephanie Cheuk Yin Yu Abstract The discovery of fetal circulating nucleic acids
in maternal plasma and serum has revolutionized prenatal genetic testing by
providing a non-invasive source of fetal genetic material. Since fetal DNA
coexists ...

Circulating Nucleic Acids in Early Diagnosis  Prognosis and Treatment Monitoring

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9401791686

Page: 471

View: 519

DNA and RNA fractions can be isolated from a variety of body fluids including whole blood, serum, plasma, urine, saliva and cerebrospinal fluid from both patients and healthy individuals. Such isolates can be exploited in the early detection of clinical disorders, stratification of patients for treatment, treatment monitoring and clinical follow-up. In addition, the use in fetal medicine allows the early detection of fetal sex, Rh factor and aneuploid disorders as well as following both fetal and premature born infant development. This volume is intended as a primer for those who are interested in entering the field of circulating nucleic acids. The areas covered in this volume include: · Background and general biology of circulating nucleic acids · Methodology · Applications of circulating nucleic acids · Quality Assurance · Ethics