Nanotechnology for Nucleic Acid Delivery

by Manfred Ogris

Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Nanotechnology for Nucleic Acid Delivery

Author: Manfred Ogris

Publisher: Humana Press

ISBN: 9781627031394

Page: 286

View: 363

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Nanotechnology for the Delivery of Therapeutic Nucleic Acids

by Dan Peer

“Nucleic acid–based therapy would have revolutionized medicine many times if
the problem of delivery had been solved, at least in small part. However, that is
proving to be as challenging a problem as any in the sciences—and of the few
with ...

Nanotechnology for the Delivery of Therapeutic Nucleic Acids

Author: Dan Peer

Publisher: CRC Press

ISBN: 9814411051

Page: 240

View: 441

Nucleic acid (NA) therapeutics has been extensively studied both in the academia and in the pharmaceutical industry and is still considered the promise for new therapeutic modalities, especially in personalized medicine. The only hurdle that limits the translation of NA therapeutics from an academic idea to the new therapeutic modality is the lack of efficient and safe delivery strategies. Nanotechnology for the Delivery of Therapeutic Nucleic Acids, written by world experts in the field of nanotechnology for NA delivery, the contributing authors bring together the state of the art in delivery strategies with strong emphasis on aspects that are of essence to the pharmaceutical industry, such as stability, general toxicity, immune-toxicity, pharmacokinetics, efficacy, and validation of new drug targets using unique approaches based on exquisite nanotechnology strategies.

Biomedical Applications of Nanotechnology

by Vinod Labhasetwar

2.2 NUCLEIC ACID DELIVERY—WHAT FOR? Nucleic acids carry the building
plans of living systems. Nucleic acid sequences are translated into structures and
functions of cellular molecules, which, together with the biochemical reactions in
 ...

Biomedical Applications of Nanotechnology

Author: Vinod Labhasetwar

Publisher: John Wiley & Sons

ISBN: 9780470152911

Page: 272

View: 800

An overview of nanotechnology and its potential The field of nanotechnology is undergoing rapid developments on many fronts. This reference provides a comprehensive review of various nanotechnologies with a view to their biomedical applications. With chapters contributed by distinguished scientists from diverse disciplines, Biomedical Applications of Nanotechnology : Reviews recent advances in the designing of various nanotechnologies based on nucleic acids, polymers, biomaterials, and metals Discusses biomedical nanotechnology in areas such as drug and gene delivery Covers advanced aspects of imaging and diagnostics Includes a chapter on the issue of nanotoxicology Complete with figures and tables, this is a practical, hands-on reference book for researchers in pharmaceutical and biotech industries, biomedical engineers, pharmaceutical scientists, pharmacologists, and materials scientists as well as for the policymakers who need to understand the potential of nanotechnology. It is also an excellent resource book for graduate-level students in pharmaceutical sciences, biomedical engineering, and other fields in which nanotechnology is playing an increasingly important role.

Nanotechnology Based Approaches for Targeting and Delivery of Drugs and Genes

by Vijay Mishra

full advantage of lung delivery, a suitable delivery system with appropriate
physicochemical properties is necessary and SLNs is most fitted on this ... Apart
from that, various biological barriers also present problems in nucleic acid
delivery.

Nanotechnology Based Approaches for Targeting and Delivery of Drugs and Genes

Author: Vijay Mishra

Publisher: Academic Press

ISBN: 0128097183

Page: 552

View: 897

Nanotechnology-Based Approaches for Targeting and Delivery of Drugs and Genes provides an overview of the important aspects of nanomedicine in order to illustrate how to design and develop novel and effective drug delivery systems using nanotechnology. The book is organized into three sections, beginning with an introduction to nanomedicine and its associated issues. Section two discusses the latest technologies in nanomedicine, while the third section covers future developments and challenges in the field. By focusing on the design, synthesis, and application of a variety of nanocarriers in drug and gene delivery, this book provides pharmaceutical and materials science students, professors, clinical researchers, and industry scientists with a valuable resource aimed at tackling the challenges of delivering drugs and genes in a more targeted manner. Explores a wide range of promising approaches for the diagnosis and treatment of diseases using the latest advances in cutting-edge nanomedical technologies Contains contributions from world-renowned experts and researchers working in the area of nanomedicine and drug delivery Covers the associated challenges and potential solutions to working with nanotechnology in drug delivery Highlights crucial topics, such as biopharmaceutical and toxicity issues, quality by design, drug targeting, and more

Nucleic Acid Nanotechnology

by Jørgen Kjems

... 108, 109 DNA nanotechnology future perspectives analysis and diagnosis,
155–156 drug delivery, 153–155 electronics and photovoltaics, 157–158
structural DNA nanotechnology (see Structural DNA nanotechnology) DNA
origami array ...

Nucleic Acid Nanotechnology

Author: Jørgen Kjems

Publisher: Springer Science & Business Media

ISBN: 3642388159

Page: 339

View: 511

This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.

RNA Nanotechnology and Therapeutics

by Peixuan Guo

RNA. Nanotechnology*. Peixuan Guo, Farzin Haque, Brent Hallahan, Randall
Reif, Hui Li, and Shaoying Wang ... 2.6.3 Nanoscale Size for Enhanced
Permeability and Retention Effects ..................38 2.6.4 Targeted Delivery to
Cancer Cells .

RNA Nanotechnology and Therapeutics

Author: Peixuan Guo

Publisher: CRC Press

ISBN: 1466505834

Page: 611

View: 550

Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in R

Chemical Biology of Nucleic Acids

by Volker A. Erdmann

RNA. Delivery. Vehicles. Using. Nucleic. Acid. Nanotechnology. Anders Hauge
Okholm, David Schaffert, and Jørgen Kjems Contents 1 Introduction.........................
......................................................... 326 2 Creating Nanostructures from DNA and ...

Chemical Biology of Nucleic Acids

Author: Volker A. Erdmann

Publisher: Springer Science & Business

ISBN: 3642544525

Page: 599

View: 833

This volume contains 29 engrossing chapters contributed by worldwide, leading research groups in the field of chemical biology. Topics include pre-biology; the establishment of the genetic code; isomerization of RNA; damage of nucleobases in RNA; the dynamic structure of nucleic acids and their analogs in DNA replication, extra- and intra-cellular transport; molecular crowding by the use of ionic liquids; new technologies enabling the modification of gene expression via editing of therapeutic genes; the use of riboswitches; the modification of mRNA cap regions; new approaches to detect appropriately modified RNAs with EPR spectroscopy and the use of parallel and high-throughput techniques for the analysis of the structure and new functions of nucleic acids. This volume discusses how chemistry can add new frontiers to the field of nucleic acids in molecular medicine, biotechnology and nanotechnology and is not only an invaluable source of information to chemists, biochemists and life scientists but will also stimulate future research.

Nucleic Acids as Gene Anticancer Drug Delivery Therapy

by Loutfy H. Madkour

This book presents the use of nanotechnology for medical applications, focusing on its use for anticancer drug delivery.

Nucleic Acids as Gene Anticancer Drug Delivery Therapy

Author: Loutfy H. Madkour

Publisher: Academic Press

ISBN: 0128197781

Page: 650

View: 732

Nucleic Acids as Gene Anticancer Drug Delivery Therapy highlights the most recent developments in cancer treatment using nucleic acids, nanoparticles and polymer nanoparticles for genomic nanocarriers as drug delivery, including promising opportunities for targeted and combination therapy. The development of a wide spectrum of nanoscale technologies is beginning to change the scientific landscape in terms of disease diagnosis, treatment, and prevention. This book presents the use of nanotechnology for medical applications, focusing on its use for anticancer drug delivery. Various intelligent drug delivery systems such as inorganic nanoparticles and polymer-based drug delivery are discussed. The use of smart drug delivery systems seems to be a promising approach for developing intelligent therapeutic systems for cancer immunotherapies and is discussed in detail along with nucleic acid-targeted drug delivery combination therapy for cancer. Nucleic Acids as Gene Anticancer Drug Delivery Therapy will be a useful reference for pharmaceutical scientists, pharmacologiests, and those involved in nanotechnology and cancer research. Discusses intelligent drug delivery systems such as inorganic nanoparticles and polymer-based drug delivery Contains a comprehensive comparison of various delivery systems, listing their advantages and limitations Presents combination therapy as a new hope for enhancing current gene-based treatment efficacy

Nanotechnology and Regenerative Engineering

by Cato T. Laurencin

... Nucleic Acid Delivery for Tissue Engineering.................................................... 19
1.4.5.1 Techniques for Gene Delivery................................................................. 19 1.4.
5.2 Major Barriers in Gene Delivery and Conventional Solutions...........20 1.4.5.3
 ...

Nanotechnology and Regenerative Engineering

Author: Cato T. Laurencin

Publisher: CRC Press

ISBN: 1466585382

Page: 476

View: 648

Nanotechnology and regenerative engineering have emerged to the forefront as the most versatile and innovative technologies to foster novel therapeutic techniques and strategies of the twenty-first century. The first edition of Nanotechnology and Tissue Engineering: The Scaffold was the first comprehensive source to explain the developments in nanostructured biomaterials for tissue engineering, the relevance of nanostructured materials in tissue regeneration, and the current applications of nanostructured scaffolds for engineering various tissues. This fully revised second edition, renamed Nanotechnology and Regenerative Engineering: The Scaffold, provides a thorough update to the existing material, bringing together these two unique areas to give a perspective of the emerging therapeutic strategies for a wide audience. New coverage includes: Updated discussion of the importance of scaffolds in tissue engineering Exploration of cellular interactions at the nanoscale Complete range of fabrication processes capable of developing nanostructured scaffolds for regenerative engineering Applications of nanostructured scaffolds for neural, skin, cardiovascular, and musculoskeletal regenerative engineering FDA approval process of nanostructure scaffolds Products based on nanostructured scaffolds Due to the unique and tissue-mimic properties of the nanostructured scaffolds, the past five years have seen a tremendous growth in nanostructured materials for biological applications. The revised work presents the current state-of-the-art developments in nanostructured scaffolds for regenerative engineering.

DNA and RNA Nanobiotechnologies in Medicine Diagnosis and Treatment of Diseases

by Volker A. Erdmann

Biomedical Applications for Nucleic Acid Nanodevices Max Scheible, Maximilian
Weitz, Andrea Mijckl, Korbinian Kapsner, ... Keywords DNA nanotechnology -
Molecular devices - Molecular programming - Controlled release - Drug delivery
M.

DNA and RNA Nanobiotechnologies in Medicine Diagnosis and Treatment of Diseases

Author: Volker A. Erdmann

Publisher: Springer Science & Business Media

ISBN: 3642368530

Page: 460

View: 676

This book will provide latest insights in the functional potentials of ribonucleic acids in medine and the use of Spiegelmer and Spiegelzyme systems. It will also deal with a new type of delivery systems for cellular targeting.

Nanobiomaterials Handbook

by Balaji Sitharaman

Seong Loong LO Institute of Bioengineering 9.1 Introduction......... d hnol and
Nanotechnology 9.2 RNA Interference. Yukti Choudhury 9.3 siRNA Delivery....
Institute of Bioengineering Physical Methods • Chemical Modification of siRNA .
and ...

Nanobiomaterials Handbook

Author: Balaji Sitharaman

Publisher: CRC Press

ISBN: 142009467X

Page: 737

View: 917

Nanobiomaterials exhibit distinctive characteristics, including mechanical, electrical, and optical properties, which make them suitable for a variety of biological applications. Because of their versatility, they are poised to play a central role in nanobiotechnology and make significant contributions to biomedical research and healthcare. Nanobio

DNA Vaccines

by W. Mark Saltzman

10 A Dendrimer-Like DNA-Based Vector for DNA Delivery A Viral and Nonviral
Hybrid Approach Dan Luo, Yougen Li, ... Key Words: Dendrimer; dendrimer-like
DNA; multivalent; hybrid vector; nucleic acid engineering; DNA nanotechnology.
1.

DNA Vaccines

Author: W. Mark Saltzman

Publisher: Springer Science & Business Media

ISBN: 1597451681

Page: 384

View: 657

Presents a comprehensive collection of DNA vaccine protocols. Divided into five sections, this volume contains state-of-the-art and practical procedures on the latest DNA vaccine technology, and demonstrates the tremendous progress that has been made in the field of DNA vaccination since the initial appearance of this new vaccination strategy.

Theory Techniques and Applications of Nanotechnology in Gene Silencing

by Surendra Nimesh

need of a delivery vehicle capable of administering siRNA efficiently, safely, and
repeatedly toin vitroandin vivomilieu. The carrier ... Nanoparticles prepared from
polycationic polymers have been largely explored to deliver DNA and siRNA.

Theory Techniques and Applications of Nanotechnology in Gene Silencing

Author: Surendra Nimesh

Publisher: River Publishers

ISBN: 8792329837

Page: 193

View: 371

Gene silencing mediated through short interfering RNAs (siRNAs) is rapidly emerging as a new class of therapeutics for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability in physiological conditions limits its therapeutic potential, hence there arises a need to develop a delivery system which can protect and efficiently transport siRNA to the target cells. Nanotechnology based non-viral vectors have been proposed as a potential alternate. Various polymeric nanoparticles have been shown as suitable delivery candidates with high cellular uptake efficiencies and advantage of reduced cytotoxicity. These delivery vectors form condensed complexes with siRNA which in turn provides protection to siRNA from enzymatic degradation and further leads to enhanced tissue and cellular targeting. Nanoparticles prepared from various polymers like polyethylenimine, chitosan have been largely exploited, as they bear several advantages such as, easy manipulatibility, high stability, low cost and high payload. Though numerous publications have appeared in the literature, there was still need to get more information under one roof, as book compilation which could provide a systematic account of all the aspects of nanotechnology mediated gene silencing right from the preparation of nanoparticles to in vitro and in vivo characterization followed by various applications in vitro and in vivo models. This book focuses on various aspects of nanotechnology based gene silencing. The initial chapters detail the techniques available for in vitro and in vivo characterization of nanoparticles. In the later chapters, exhaustive details about various polymeric systems employed for gene silencing has been accounted. The important topics discussed in the book include: Introduction to Nanotechnology Physicochemical Characterization of Nanoparticles In Vitro and In Vivo Characterization of Nanoparticles Concept and Barriers to Gene Silencing Nanoparticles Mediated Targeted siRNA delivery Polymers for siRNA Delivery Chitosan Polyethylenimine Poly-L-lysine Atelocollagen Protamine Dendrimers Cyclodextrin Poly (D, L-lactide-co-glycolide) Nanomaterials for siRNA delivery

RNA Nanotechnology

by Bin Wang

Small interfering RNA against the 2C genomic region of coxsackievirus B3 exerts
potential antiviral effects in ... Targeted delivery of mutant tolerant
anticoxsackievirus artificial microRNAs using folate conjugated bacteriophage
Phi29 pRNA.

RNA Nanotechnology

Author: Bin Wang

Publisher: CRC Press

ISBN: 9814411655

Page: 466

View: 307

In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.

Nanotechnology for Cancer Therapy

by Mansoor M. Amiji

16. Aptamers. and. Cancer. Nanotechnology. Omid C. Farokhzad, Sangyong Jon,
and Robert Langer CONTENTS 16.1 Targeted Drug Delivery: An Introduction
16.2 Nucleic Acid Ligands (Aptamers): From Discovery to Practice .

Nanotechnology for Cancer Therapy

Author: Mansoor M. Amiji

Publisher: CRC Press

ISBN: 1420006630

Page: 840

View: 726

While simultaneous breakthroughs occurring in molecular biology and nanoscience/technology will ultimately revolutionize all of medicine, it is with our efforts to prevent, diagnose, and treat cancer that many of the most dramatic advances will occur. In support of this potential, the U.S. National Cancer Institute (NCI) established the Alliance fo

Carbohydrate Chemistry

by Tiina Alamäe

Emphasis will be laid on the synergic combination of chemical synthesis and
nanotechnology to correlate architectural features of the glycotransporters with
nucleic acid delivering capabilities, feeding back newly optimized designs.
Though ...

Carbohydrate Chemistry

Author: Tiina Alamäe

Publisher: Royal Society of Chemistry

ISBN: 1849734399

Page: 445

View: 146

Annotation 'Carbohydrate Chemistry' provides review coverage of all publications relevant to the chemistry of monosaccharides and oligosaccharides in a given year.

Protein Nanotechnology

by Tuan Vo-Dinh

13 Bacterial Virus φ29 DNA-Packaging Motor and Its Potential Applications in
Gene Therapy and Nanotechnology Peixuan ... and size make this RNA-
containing motor a promising tool for drug and gene delivery and for use in
nanodevices.

Protein Nanotechnology

Author: Tuan Vo-Dinh

Publisher: Springer Science & Business Media

ISBN: 1592598587

Page: 463

View: 683

Leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens.

Multifaceted Development and Application of Biopolymers for Biology Biomedicine and Nanotechnology

by P.K. Dutta

Gene delivery has been classically achieved by a variety of methods that use a
viral or a non-viral vector packaged with the nucleic acid of interest. In the last
decade, several newer approaches to gene delivery have emerged that utilize ...

Multifaceted Development and Application of Biopolymers for Biology Biomedicine and Nanotechnology

Author: P.K. Dutta

Publisher: Springer

ISBN: 3642401236

Page: 346

View: 144

Nanoparticles for Gene Delivery into Stem Cells and Embryos, by Pallavi Pushp, Rajdeep Kaur, Hoon Taek Lee, Mukesh Kumar Gupta. Engineering of Polysaccharides via Nanotechnology, by Joydeep Dutta. Hydroxyapatite-Packed Chitosan-PMMA Nanocomposite: A Promising Material for Construction of Synthetic Bone, by Arundhati Bhowmick, Subhash Banerjee, Ratnesh Kumar, Patit Paban Kundu. Biodegradable Polymers for Potential Delivery Systems for Therapeutics, by Sanjeev K. Pandey, Chandana Haldar, Dinesh K. Patel, Pralay Maiti. Phytomedicine-Loaded Polymeric Nanomedicines: Potential Cancer Therapeutics, by S. Maya, M. Sabitha, Shantikumar V. Nair, R. Jayakumar. Proteins and Carbohydrates as Polymeric Nanodrug Delivery Systems: Formulation, Properties and Toxicological Evaluation, by Dhanya Narayanan, J. Gopikrishna, Shantikumar V. Nair, Deepthy Menon. Biopolymeric Micro and Nanoparticles: Preparation, Characterization and Industrial Applications, by Anil Kumar Anal, Alisha Tuladhar. Applications of Glyconanoparticles as “Sweet” Glycobiological Therapeutics and Diagnostics, by Naresh Kottari, Yoann M. Chabre, Rishi Sharma, René Roy.